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imagelab 5 2 1 software  (Bio-Rad)


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    Structured Review

    Bio-Rad imagelab 5 2 1 software
    Imagelab 5 2 1 Software, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1603 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/imagelab+5+2+1/Image+Lab+Software+for+Mac+Version+5%2E2%2E1/pm41863024-75-26-29
    Average 99 stars, based on 1603 article reviews
    imagelab 5 2 1 software - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Software:

    Article Title: Clusterin can mediate apoptosis-induced molecular mechanisms in immune thrombocytopenia
    Article Snippet: To detect the signal intensity after adding chemiluminescent reagents we used the ChemiDoc Touch Imaging System (Biorad, CA, USA). .. For the analysis we used Biorad ImageLab 5.2.1. software. ..


    Article Title: Effects of Exercise on Urinary Parameters and Proteins in Well-Trained Canicross Dogs: A Preliminary Study
    Article Snippet: The gels were stained with Coomassie brilliant blue (PageBlu protein staining solution; Thermo Fisher Scientific, Waltham, MA, USA). .. After staining, each gel was digitalized (ChemidocMP, BioRad, Hercules, CA, USA), and pherograms were obtained using commercial software (ImageLab 5.2.1, BioRad, Hercules, CA, USA). .. The concentration of uromodulin and albumin in the respective bands was calculated using an internal standard of quantity as reported in [ ] and their ratios with creatinine (uUC and uAC), were calculated using the following formulas: uUC (without unit) = urinary uromodulin (mg/dL)/uCr (mg/dL) uAC (without unit) = urinary albumin (mg/dL)/uCr (mg/dL) To better separate the low molecular mass (LMM) bands, two μg of protein for each sample were also loaded on 12% polyacrylamide gel in MOPS buffer with SDS (Thermo Fisher Scientific, Waltham, MA, USA) and electrophoresis was carried out following the procedure reported previously; gels were stained with silver nitrate (SilverQuest; Thermo Fisher Scientific).

    Article Title: A robust and efficient microvascular isolation method for multimodal characterization of the mouse brain vasculature
    Article Snippet: After three additional washes, the antibody signal was captured with Super Signal West PICO Plus Chemiluminescence Substrate (ThermoFisher) using Gel Doc (Bio-Rad). .. The images were analyzed with ImageLab 5.2.1. software (Bio-Rad). ..

    Immunolabeling:

    Article Title: Acute restraint stress regulates brain DNMT3a and promotes defensive behaviors in male rats.
    Article Snippet: The membranes were subsequently incubated with PBS-T containing BSA 1 % and secondary HRP (horseradish peroxidase)-conjugated antibodies (anti-rabbit, 1:10000; Abcam, ab6721; and anti-mouse 1:10000; Merck, 12–349) for 2 h in room temperature. .. We used a chemiluminescent kit (SuperSignal Chemiluminescent Substrates, Thermo Fisher Scientific) to detect the immunolabeled bands in the membranes, and the ImageLab 5.2.1 (BioRad) software for densitometric analysis. ..

    Article Title: Acute restraint stress regulates brain DNMT3a and promotes defensive behaviors in male rats
    Article Snippet: The membranes were subsequently incubated with PBS-T containing BSA 1 % and secondary HRP (horseradish peroxidase)-conjugated antibodies (anti-rabbit, 1:10000; Abcam, ab6721; and anti-mouse 1:10000; Merck, 12–349) for 2 h in room temperature. .. We used a chemiluminescent kit (SuperSignal Chemiluminescent Substrates, Thermo Fisher Scientific) to detect the immunolabeled bands in the membranes, and the ImageLab 5.2.1 (BioRad) software for densitometric analysis. ..

    Staining:

    Article Title: Effects of Exercise on Urinary Parameters and Proteins in Well-Trained Canicross Dogs: A Preliminary Study
    Article Snippet: The gels were stained with Coomassie brilliant blue (PageBlu protein staining solution; Thermo Fisher Scientific, Waltham, MA, USA). .. After staining, each gel was digitalized (ChemidocMP, BioRad, Hercules, CA, USA), and pherograms were obtained using commercial software (ImageLab 5.2.1, BioRad, Hercules, CA, USA). .. The concentration of uromodulin and albumin in the respective bands was calculated using an internal standard of quantity as reported in [ ] and their ratios with creatinine (uUC and uAC), were calculated using the following formulas: uUC (without unit) = urinary uromodulin (mg/dL)/uCr (mg/dL) uAC (without unit) = urinary albumin (mg/dL)/uCr (mg/dL) To better separate the low molecular mass (LMM) bands, two μg of protein for each sample were also loaded on 12% polyacrylamide gel in MOPS buffer with SDS (Thermo Fisher Scientific, Waltham, MA, USA) and electrophoresis was carried out following the procedure reported previously; gels were stained with silver nitrate (SilverQuest; Thermo Fisher Scientific).



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